Getting Started

Run your first analysis

No account required. Cached results return in seconds.

1. Open the analyzer

Click Launch foldfunc from the homepage. Use Single mode for one protein or switch to Batch to analyze up to 20 sequences at once.

2. Input fields

Protein sequence

Required

A protein sequence in single-letter amino acid format. Spaces, newlines, and carriage returns are stripped automatically. Only the 20 standard amino acids are accepted (A C D E F G H I K L M N P Q R S T V W Y). In Batch mode, paste multiple sequences in FASTA format — each entry begins with a >Name line followed by the sequence on the next line.

MKTIIALSYIFCLVFA...

Minimum 10 residues. Maximum 2000 residues.

Protein name

Optional

A common name or identifier for the protein (e.g. "Lysozyme", "BRCA1"). Used to search for relevant literature and provides context to the AI interpretation. Leave blank if unknown.

Lysozyme C

Mutation positions

Optional

Residue positions you want targeted mutation scoring for, entered as a comma-separated list of integers. Positions are 1-indexed (the first residue is position 1). If left blank, foldfunc automatically selects positions across the full sequence.

14, 35, 52, 101

3. What you get back

3D structure viewer: An interactive molecular viewer showing the predicted fold. Toggle between Rainbow (N→C colouring) and Mutations (residue-level mutational sensitivity) colour modes.
pLDDT score: Mean per-residue confidence (0–100). Ranges from 0–100 — higher is better.
Mutation heatmap: A full-sequence colour strip showing mutational tolerance at every scored position. Click any position to expand all 20 amino acid scores and the top 5 suggested substitutions.
AI biological interpretation: A structured summary covering protein family, structural observations, mutation-sensitive positions, and open research questions.
Literature context: Up to 3 relevant abstracts retrieved for your protein name, used to ground the AI interpretation.